Plant Transcription Factor Database
Previous version: v3.0
Vigna radiata
HSF Family
Species TF ID Description
Vradi0161s00040.1HSF family protein
Vradi0246s00340.1HSF family protein
Vradi03g01100.1HSF family protein
Vradi03g02400.1HSF family protein
Vradi03g04270.1HSF family protein
Vradi06g02880.1HSF family protein
Vradi06g15090.1HSF family protein
Vradi07g03150.1HSF family protein
Vradi07g10520.1HSF family protein
Vradi07g16260.1HSF family protein
Vradi08g00250.1HSF family protein
Vradi08g02500.1HSF family protein
Vradi08g06500.1HSF family protein
Vradi08g09150.1HSF family protein
Vradi08g19000.1HSF family protein
Vradi08g19520.1HSF family protein
Vradi10g02130.1HSF family protein
Vradi10g09840.1HSF family protein
Vradi11g01010.1HSF family protein
Vradi11g02310.1HSF family protein
Vradi11g04790.1HSF family protein
Vradi11g08720.1HSF family protein
HSF Family Introduction

Heat stress transcription factors (Hsfs) are the major regulators of the plant heat stress (hs) response. Sequencing of the Arabidopsis genome revealed the existence of 21 open-reading frames (ORFs) encoding putative Hsfs assigned to classes A-C. Here we present results of a functional genomics approach to the Arabidopsis Hsf family focused on the analysis of their C-terminal domains (CTDs) harboring conserved modules for their function as transcription factors and their intracellular localization. Using reporter assays in tobacco protoplasts and yeast as well as glutathione-S-transferase (GST) pull-down assays, we demonstrate that short peptide motifs enriched with aromatic and large hydrophobic amino acid (aa) residues embedded in an acidic surrounding (AHA motifs) are essential for transcriptional activity of class A Hsfs. In contrast to this, class B and C Hsfs lack AHA motifs and have no activator function on their own. We also provide evidence for the function of a leucine (Leu)-rich region centered around a conserved QMGΦL motif at the very C-terminus as a nuclear export signal (NES) of class A Hsfs. Sequence comparison indicates that the combination of a C-terminal AHA motif with the consensus sequence FWxxF/L,F/I/L as well as the adjacent NES represents a signature domain for plant class A Hsfs, which allowed to identify more than 60 new Hsfs from the expressed sequence tag (EST) database.

Kotak S, Port M, Ganguli A, Bicker F, von Koskull-Doring P.
Characterization of C-terminal domains of Arabidopsis heat stress transcription factors (Hsfs) and identification of a new signature combination of plant class A Hsfs with AHA and NES motifs essential for activator function and intracellular localization.
Plant J, 2004. 39(1): p. 98-112.
PMID: 15200645